Real-Time PCR Applications Guide - Bio-Rad

2.1 General Considerations 8 ... Multiplexing Considerations 28 ... 5.4 qPCR Assay Development 50 5.5 Experimentation 51 5.5.1 Reaction Components for Multiplex Assay 51 5.5.2 Cycling Protocol 51 5.6 Gene Expression Data Analysis 52 6. Genotyping/Allelic Discrimination 54

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