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2）培養細胞からの RNA の回収と cDNA の合成操作

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2）TRIzol® Reagent 1mL に対して，0.2mL のクロロホルム を加える． 3）チューブのフタをしっかり閉め，15 秒間手で 激しく振って撹拌し，室温で2-3 分間静置する． 5）遠心分離後，無色透明の上層（水相）と 白色の中間相，赤色の下層（有機相）に

  Trizol

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molecular size. TRIzol™ Reagent maintains the integrity of the RNA due to highly effective inhibition of RNase activity while disrupting cells and dissolving cell components during sample homogenization. TRIzol™ Reagent allows for simultaneous processing of a large number of samples, and is an improvement to the single-step RNA isolation

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