BASIC PRACTICAL MICROBIOLOGY

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BASIC PRACTICAL MICROBIOLOGY A MANUAL Compiled by John Grainger, Chairman of MISAC Janet Hurst, Deputy Executive Secretary of SGM Dariel Burdass, SGM Educational Projects Administrator BASIC PRACTICAL MICROBIOLOGY : A MANUAL The Society for General MICROBIOLOGY 2001 Contents Part 1: THE basics An introduction to MICROBIOLOGY , aseptic technique and safety Page Number PREPARATION Safety guidelines 1 Risk assessment 1 Good microbiological laboratory practice (GMLP) 3 Spillage management 3 Aerosols 4 RESOURCES Equipment 4 Apparatus 5 Materials 5 MEDIA, STERILIZATION AND DISINFECTANTS Preparation of culture media 6 Storage of media 6 Sterilization vs.

Small volumes (ca 5-10 cm3) of liquid media/agar slopes/sterile solutions for inoculation (held in test tube rack; dry non-absorbent cotton wool plug or plastic cap prevents contamination) Universal bottle (wide neck); McCartney bottle (narrow neck) Volumes of liquid and agar media/sterile solutions up to ca 20 cm3 for inoculation or for

  Basics, Microbiology, Practical, Basic practical microbiology