Chapter 5 - Bacteriology - FWS

← Back to document page

NWFHS Laboratory Procedures Manual - Second Edition, June 2004 Chapter 5 - Page 1 Chapter 5 Bacteriology Jason Woodland USFWS - Pinetop Fish Health Center Pinetop, Arizona NWFHS Laboratory Procedures Manual - Second Edition, June 2004 Chapter 5 - Page 2 I. Introduction This section defines the procedures and techniques used to correctly identify the target bacterial pathogens identified for the Survey (Yersinia ruckeri, Aeromonas salmonicida and Edwardsiella ictaluri). Proper identification relies on bacterial growth characteristics, appropriate biochemical tests, and corroboration by serological techniques. Pathogens of Regional Importance (PRIs) include: Citrobacter freundii, Edwardsiella tarda, Flavobacterium columnare and Flavobacterium psychrophilum.

3% potassium hydroxide (KOH) solution (3 grams KOH per 100 mL distilled water) on a clean glass slide. B. Stir for about one minute, occasionally lifting the loop to look for thickening and “stringing” of the slurry. C. RESULTS: 1. Gram positive bacteria will not appear to change the viscosity of the KOH solution. 2.

  Solutions, Chapter, Bacteriology, Chapter 5 bacteriology