Column Protection Guide Version 0610 - Phenomenex

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Column Protection GuideVersion 0610Includes: Mobile Phase Limitations Column Storage Tips Column Protection DevicesCoLuMn Protection guiDeTABLe oF ConTenTSINTRODUCTION TO Column CARE AND USAGE 1-4Selecting the Right Tubing and FittingsColumn InstallationPART I - SILICA-BASED & TWIN TECHNOLOGY columns 4-7Running ParametersScaling Up/Scaling DownMobile Phase ConsiderationsColumn StorageStationary Phase ConsiderationsColumn Cleaning ProceduresBackpressure and Flow RatesPART II - SFC (SUPERCRITICAL FLUID CHROMATOGRAPHY) 7Running ParametersCleaning ProcedureEquilibrating ColumnColumn StorageMobile Phase ConsiderationsPART III -AXIA PREPARATIVE columns 8Running ParametersCleaning ProcedureMobile Phase ConsiderationsColumn StoragePART IV - LUX CHIRAL columns 8-9Running ParametersExtending Lifetime and ReconditioningMobile Phase ConsiderationsColumn StoragePART V - CHIREX CHIRAL columns 10Running ParametersMobile Phase ConsiderationsPART VI - BIOSEP-SEC-S columns 10Running ParametersMobile Phase ConsiderationsCleaning ProcedureColumn StoragePART VII - REZEX POLYMER-BASED columns 11-15Running ParametersMobile Phase ConsiderationsCleaning ProcedureTable of Specifications and Operating ParametersPART VIII - POLYSEP-GFC-P columns 16Running ParametersMobile Phase ConsiderationsCleaning ProcedureColumn StoragePART IX - PHENOGEL GPC columns 17, 20-21SpecificationsSolvent Switching ConsiderationsSample ConsiderationsSolvent Compatibilit

1 inTroDuCTion Every Phenomenex HPLC column is a precision product which, though delicate, will provide excellent performance, reproducibility and column lifetime if cared for properly.

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