Droplet Digital Applications Guide

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Droplet Digital PCRDroplet Digital PCR Applications GuideDroplet Digital PCR Applications Guide | iTable of ContentsChapter 1 Droplet Digital PCR . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1QX100/QX200 Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2Droplet Generation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3PCR Amplification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4Droplet Reading.

6oplet Digital PCR Applications Guide | Dr Droplet Digital PCR Droplet Digital PCR data from a duplex experiment in which two targets are PCR amplified can also be viewed in a 2-D plot in which channel 1 fluorescence (FAM) is plotted against channel 2 fluorescence (HEX or VIC) for each droplet (Figure 1.10).

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