Flow Cytometry Basics Guide - Bio-Rad

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Flow CytometryPractical Advice to Get You Started in FlowFlow Cytometry Basics GuideVisit for more information Flow Cytometry Basics Guide | 1Table of ContentsChapter 1 Principles of the Flow CytometerFluidics System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3Optics and Detection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4Signal and Pulse Processing.

laser beam or beams and the signal generated at each point in the cell’s path . These pulses can be mapped by plotting signal as a function of time . As the particle enters the laser beam spot, it will generate scattered light and fluorescence signals, which will ultimately manifest in a stream of electrons (current) from the anode of the PMT .

  Basics, Laser