for FLUORESCENCE MICROSCOPY

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CHROMA TECHNOLOGY CORPHANDBOOK ofOPTICAL FILTERSfor FLUORESCENCEMICROSCOPYby JAY REICHMAN 2017AN INTRODUCTION TOFLUORESCENCE MICROSCOPY 2Excitation and emission spectraBrightness of the FLUORESCENCE signalThe FLUORESCENCE microscopeTypes of filters used in FLUORESCENCE microscopyThe evolution of the FLUORESCENCE microscopeA GENERAL DISCUSSION OF OPTICAL FILTERS 8TerminologyAvailable products Colored filter glass Thin-film coatings Acousto-optical filters Liquid Crystal Tunable FiltersDESIGNING FILTERS FORFLUORESCENCE MICROSCOPY 14Image Contrast FLUORESCENCE spectra Light sources Detectors BeamsplittersOptical quality Optical quality parameters Optical quality requirements for wide-field microscopyFILTER SETS FOR SUB-PIXEL REGISTRATION 24FILTERS FOR CONFOCAL MICROSCOPY 25Optical quality requirements Nipkow-disk scanning Laser scanningSpectral requirements Nipkow-disk scanning Laser scanningFILTERS FOR MULTIPLE PROBE APPLICATI

Fluorescence microscopy requires optical filters that have demanding spectral ... This guide is a compilation of the principles and know-how that the engineers ... • Raman spectroscopy. Our coating lab and optics shop are integrated into a single facility operated

  Principles, Spectroscopy, Fluorescence