for FLUORESCENCE MICROSCOPY - Chroma
Chroma TECHNOLOGY CORPHANDBOOK ofOPTICAL FILTERSfor FLUORESCENCEMICROSCOPYby JAY REICHMAN 2017AN INTRODUCTION TOFLUORESCENCE MICROSCOPY 2Excitation and emission spectraBrightness of the FLUORESCENCE signalThe FLUORESCENCE microscopeTypes of filters used in FLUORESCENCE microscopyThe evolution of the FLUORESCENCE microscopeA GENERAL DISCUSSION OF OPTICAL FILTERS 8TerminologyAvailable products Colored filter glass Thin-film coatings Acousto-optical filters Liquid Crystal Tunable FiltersDESIGNING FILTERS FORFLUORESCENCE MICROSCOPY 14Image Contrast FLUORESCENCE spectra Light sources Detectors BeamsplittersOptical quality Optical quality parameters Optical quality requirements for wide-field microscopyFILTER SETS FOR SUB-PIXEL REGISTRATION 24FILTERS FOR CONFOCAL MICROSCOPY 25Optical quality requirements Nipkow-disk scanning Laser scanningSpectral requirements Nipkow-disk scanning Laser scanningFILTERS FOR MULTIPLE PROBE APPLICATIONS 29REFERENCES 30GLOSSARY 31CHROMA TECHNOLOGY CORPHANDBOOK ofOPTICAL FILTERSfor FLUORESCENCEMICROSCOPYby JAY REICHMAN 1Fluorescence MICROSCOPY requires optical filters that have demanding spectral and physical characteristics.
Camera Fluorescence Filter Cube Field Stop Aperature Stop Objective Specimen Stage Arc Lamp Collector Lenses Heat Filter Eyepiece Illumination Path Imaging Path Filter Slider Filter Slider FIGURE 3 Schematic of a wide-field epifluorescence microscope, showing the separate optical paths for illuminating the specimen and imaging the specimen ...
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