遺伝子発現に重要なベクターの要素 - lonzabio.jp
1 Amaxa . . CMV . . CMV . 1 . CAT . . 100% CMV . 10 . . . . . BHK-21 . 1 . SV40 . . . 10 . . . . mRNA . . . . A. 1600. Relative luciferase-expression % of [pGL3-CMV at 24 h]. 1400. 1200. 1000. 800. 600. 400. 200. 0. Control Program pGL3- pGL3-CMV pmax pSG5 pFP pIRES- pcDNA4 pCMVbeta pCMV-TNT. only control Cloning MCS B HisMax B. 2000. Relative luciferase-expression % of [pGL3-CMV at 24 h]. 1500. 1000. 500. 0. Control Program pGL3- pGL3-CMV pmax pSG5 pFP pIRES- pcDNA4 pCMVbeta pCMV-TNT. only control Cloning MCS B HisMax 4 hours 24 hours 48 hours 1. Luciferase expression levels depend on vector backbones.
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