Method Development Guide (rev. 05/04) - ZirChrom

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ZirChrom -EZ & ZirChrom -MSMethodDevelopmentGuidetel1-8866-SSTAB LE-11/fax1-7763-4421-22319 -EZ & ZirChrom -MSMethodDevelopmentGuideColumn Use TipsLewisAcidSite Deactivated Zirconia-BBasedColumnsforHPLCIncludesNEW Quick-Start Guide !PART I / QUICK-SSTARTGUIDEStart-uupProcedureX2Ins tallingtheColumnValidatingColumnPerforma nceMobilePhasepHEquilibratingtheColumnUs ewithLC/MSZirChrom -EEZ& ZirChrom -MMSX4GeneralUseTipsColumnMaintenanceX5C leaning/RegenerationProcedureStorageCond itionsPART you for purchasing a ZirChrom hplc column. Due to its unique characteristics namely that its packing material is zirconia-based rather than silica-based, we strongly recommend that you read this Guide beforeusing your column. Method Development with zirconia-based columnsinvolves different steps than those used with silica- or polymer-based this Guide , we have outlined those steps and different chemistries. If at anytime you have questions about your column or Method Development , pleasecall our technical support line at 1-8866-SSTABLE-11(1-8866-7782-22531) to speak toone of our on-staff chemists, or email us at will be happy to help : This Guide is for ZirChrom -EZ and ZirChrom -MS only.

Pleasereadthisguidecarefullybeforeusingyournewcolumn. INTRODUCTION Thank you for purchasing a ZirChrom® HPLC column. Due to its …

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