PCR Optimization Student Guide 2012 - Babec

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PCR Optimization Table of Contents Fall 2012 Optimizing the Polymerase Chain Reaction Introduction ........................................ ........................................ ........................................ ..1 Review of Mathematics ........................................ ........................................ ...........................3 Solving Problems of dilution and Concentration: Two Approaches ........................................ ..........4 Experiment Overview ........................................ ........................................ ............................7 Calculations Part 1: Overview of PCR Reaction Components ........................................ ........................8 Part 2: Varying the Magnesium Chloride Concentration ........................................ .............10 Part 3: Varying the Primer Concentration ........................................ ...............................15 Laboratory Important Laboratory Practices.

PCR Optimization Student Guide Fall 2012 5 ! Solving Problems of Dilution and Concentration: Two Approaches Everyone has a slightly different manner in which they think about numbers and mathematical relationships.

  Dilution