Reversed Phase Chromatography

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Reversed PhaseChromatographyPrinciples and Methods18-1134-16Edition AAReversed PhaseChromatographyContents1. Introduction ........................................ ........................................ .............. 5Theory of Reversed Phase Chromatography ........................................ ....... 6The matrix ........................................ ........................................ ................ 9The ligands ........................................ ........................................ ............. 11Resolution in Reversed Phase Chromatography ........................................ 13Resolution ........................................ ........................................ .............. 13Capacity factor.

6 Theory of reversed phase chromatography The separation mechanism in reversed phase chromatography depends on the hydrophobic binding interaction between the solute ...

  Phases, Chromatography, Reserved, Reversed phase chromatography