SEPARATION TECHNIQUES - uspbpep.com
Chromatographic SEPARATION techniquesEUROPEAN PHARMACOPOEIA , by passing the pressurised solvent through apulse-damping device. Tubing and connections are capableof withstanding the pressures developed by the controlled systems are capable of accuratelydelivering a mobile phase in either constant or varyingconditions, according to a defined programme. In the case ofgradient elution, pumping systems which deliver solvent(s)from several reservoirs are available and solvent mixing canbe achieved on either the low or high-pressure side of thepump(s).InjectorsInjection may be carried out directly at the head of thecolumn using a phasesStationary phases are contained in columns which havebeen described in the chapters onLiquid chromatography( ) (packed columns) andGas chromatography( )(capillary columns).
2.2.46. Chromatographic separation techniques EUROPEAN PHARMACOPOEIA 6.0 minimised, e.g. by passing the pressurised solvent through a pulse-damping device. Tubing and connections are capable
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