The Bacterial Reverse Mutation Test

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CHAPTER 4The Bacterial Reverse Mutation TestAnnie Hamel1, Marise Roy1and Ray Proudlock21Department of Genetic Toxicology, Charles River Laboratories, Montreal, ULC, Canada2Boone, North Carolina, USAChapter Introduction History Fundamentals Equipment Consumables Reagents and Recipes Ampicillin 2 g/disc Biotin mg/mL Crystal Violet 5 g/disc Glucose g/mL 1M: Glucose-6-Phosphate : 500 M Histidine, 500 M Biotin, 500 M Tryptophan Solution Histidine 5 mg/mL KMg MGA Plates Minimal Glucose Master (MGM, MGMA and MGMAT) Plates NADP M Nutrient Agar Plates Nutrient Broth Phosphate Buffer M pH Positive Control and Diagnostic Mutagen Solutions S9 Fraction S9 Mix Tetracycline 1 g/disc Top Agar Incomplete: TAI Top Agar Complete: TAC Tryptophan 5 mg/mL VB Salts 503: Vogel-Bonner Salts Suggested Phases in Development of the Test The Bacterial Strains Genotypes of Routinely Used Strains Obtaining the T

4.9.2 Test Article Considerations 106 4.9.2.1 Solvent selection 109 4.9.2.2 Dose volumes 109 4.9.2.3 Dose levels 109 4.9.3 Positive Controls 113 4.10 Standard Test Procedures 114 4.10.1 Plate Incorporation Method 114 4.10.2 Preincubation Method 115 4.10.3 Standard Study Design 116 4.10.4 Examination of the Plates 118 4.10.5 Interpretation of ...

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