The simultaneous saccharification and …
G)Copyright1991by The HumanaPress Inc. All rights of any nature whatsoeverreserved. 0273-2289/91 The simultaneous saccharification and fermentation of Pretreated Woody Crops to Ethanol DIANE D. SPINDLER,* CHARLES E. WYMAN, AND KAREL GROHMANN. Biotechnology Research Branch, Fuels and Chemical Research and Engineer Diuision, Solar Energy Research Institute, 16I 7 Cole Boulevard, Golden, CO ABSTRACT. Four promising woody crops (Populus maximowiczii x nigra (NE388), P. trichocarpa x deltoides (Nll), P. tremuloides, and Sweetgum Liquidambar styraciflua) were pretreated by dilute sulfuric acid and evaluated in the simultaneous saccharification and fermentation (SSF). process for ethanol production. The yeast Saccharomyces cerevisiae was used in the fermentations alone, and in mixed cultures with/~-gluco- sidase producing Brettanomyces clausenii. Commercial Genencor 150L.
774 Spindler, Wyman, and Grohmann INTRODUCTION The simultaneous saccharification and fermentation (SSF) process for conversion of cellulose into ethanol was first studied by Takagi et al. (1,2)
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