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Thin Layer Chromatography (TLC) Guide

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thin Layer Chromatography (TLC) Guide Overview: thin Layer Chromatography (TLC) is an extremely useful technique for monitoring reactions. It is also used to determine the proper solvent system for performing separations using column Chromatography . TLC uses a stationary phase, usually alumina or silica, that is highly polar (standard) or non-polar (reverse phase), and a mobile phase, some solvent whose polarity you will choose. In , and in most lab applications, you will use standard phase silica plates. You will apply your reaction mixture in solution to the plate then "run" the plate by allowing a solvent (or combination of solvents) to move up the plate by capillary action.

Visualize the TLC using non-destructive technique(s). The best non-destructive method is the UV lamp. Place your plate under the UV lamp and circle any UV active spots with your pencil. Although we won't do this in 5.301, another popular non-destructive method is staining with iodine. (You might see this in your UROP.) 59

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