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Page 1 of 4 1 March 22, 2006; Revised January 26, 2011 for CHMY 374 Adapted for CHMY 362 November 13, 2011 Edited by Lauren Woods December 2, 2011 Benzene version January 23, 2013, ; 18mar14, ; 2feb15 P. Callis Version 1feb17 P. Callis Experimental Procedure and Lab Report Instructions for introduction to fluorescence Spectroscopies I. Patrik Callis and Karl Sebbey (Partly Adapted from Clarke RJ, Oprysa, A. fluorescence and Light Scattering J. Chem. Ed. 81(5) 705-707 2004 1. introduction This document is the experiment part going with introduction to fluorescence Spectroscopies I. Theory Objectives: Gain a working familiarity with the Horiba Fluoromax4C Fluorometer, including making observations of 1.)

Quenching of fluorescence is the basis for many investigations using fluorescence. The introduction provided in the following is also instructive of basic first and second order kinetics, which you will be studying in Lecture. We will use the equations and data from the Theory handout.

  Introduction, Fluorescence

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