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Introduction to Fluorescence Spectroscopies I. Theory

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Page 1 of 9 March 22, 2006; Revised January 26, 2011 for CHMY 374 Adapted for CHMY 362 November 13, 2011 Edited by Lauren Woods December 2, 2011 17mar14, ; 1feb17 P. Callis Introduction to Fluorescence Spectroscopies I. Theory Patrik Callis and Karl Sebbey (Adapted from Clarke RJ, Oprysa, A. Fluorescence and Light Scattering J. Chem. Ed. 81(5) 705-707 2004) All life appears to be nurtured by the excitation of electrons by light in photosynthesis. The vision enjoyed by higher life forms begins with the electronic excitation of a conjugated polyene.

fluorescence intensity emitted by the fluorophore because of electron transfer to or from another molecule (called the quencher) to or from the fluorescing molecule, or transfer of the fluorophore’s excitation energy to another molecule.

  Introduction, Fluorescence, Introduction to fluorescence

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Quenching of fluorescence is the basis for many investigations using fluorescence. The introduction provided in the following is also instructive of basic first and second order kinetics, which you will be studying in Lecture. We will use the equations and data from the Theory handout.

  Introduction, Fluorescence

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