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Column Selection Gc

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CHAPTER 1 2 3 Introduction, Chromatography Theory, and ...

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10 hydrocarbons, the column phase should be non-polar (interacting with the 11 analyte via van der Waals forces). Selection of a similar phase with similar 12 intermolecular forces will allow more interaction between the separation column 13 and structurally similar analytes and increase their retention time in the column.

  Selection, Columns

HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)

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Column Role is to separate the components of a mixture Column – Cont. Much shorter than columns used in GC --- Why? Highly efficient separations achieved in HPLC due to interactions of both m.p. and s.p. with the components of a mixture vs. GC, where only the s.p. interacts with components No need for long columns

  Columns

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