Techniques for epigenetic analysis - Harvard University

Workflow for DNA methylation analysis by bisulfite‐pyrosequencing Biospecimen DNA isolation Bisulfite treatment PCR amplification Data analysis methylated C (methylated C) +(unmethylated C) ×100% Abbreviations: %5mC, percentage of 5-methylcytosine; C, cytosines %5mC = Pyrosequencing

  Amplification, Pcr amplification