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Prepare Sterilize And Dispense Culture Media
Found 2 free book(s)STAPHYLOCOCCUS AUREUS (CULTURE METHOD)
corn.orgMEDIA AND REAGENTS 1. Tryptic Soy Broth. Prepare medium according to the manufacturer's directions. Dispense 100 mL into 250 mL Erlenmeyer flasks. Sterilize by autoclaving at 121°C for 15 mins. 2. Use one of the following: a. Baird-Parker Agar Base. Prepare medium according to manufacturer's directions. Sterilize by autoclaving at 121°C for ...
HEK293 cells (Human embryonal kidney cells (HEK293, …
2018.igem.orgPrepare a new flask with fresh medium. Block trypsinization by adding a few ml of medium. Take a fraction of the cell solution and inocculate the new flask. Typically, when splitting confluent HEK 293 cells in a 1:10 ratio, confluency is reached again …