Direct-zol™ RNA Miniprep Plus - Zymo Research

May 11, 2021 · Sequencing, RT-qPCR, etc. ... The following guidelines are provided for processing various sample types in TRIzol ... ü Perform all steps at room temperature and centrifugation at 10,000-16,000 x g for 30 seconds, unless specified. 1. Add an equal volume ethanol (95-100%) to a sample lysed in TRI ...

  Guidelines, Temperatures, Room, Qpcr, Room temperature