Sybr Green Master Mix
Found 3 free book(s)QuantStudio 3 and QuantStudio 5 Real-Time PCR Systems
tools.thermofisher.comoptimized master mixes, enable outstanding specificity and sensitivity. Applied Biosystems™ SYBR™ Green chemistry is an economical alternative for target identification or initial screening assays. The QuantStudio 3 system has 4 filters calibrated for FAM™/SYBR Green, VIC™/JOE™, NED™/ TAMRA™, and ROX™ dyes. The QuantStudio 5 ...
Guide to Performing Relative Quantitation of Gene ...
assets.thermofisher.com• The use of TaqMan® Universal PCR Master Mix or SYBR® Green I PCR Master Mix (provides standardized component concentrations and simplifies assay set-up) • Universal thermal cycling parameters (enables multiple assays to be run on the same plate) • Default primer and probe concentrations (to eliminate assay optimization).
4. Designing and validating primers
stmichaelshospitalresearch.caReagent [Stock] Volume/rxn samples Master Vol. Final concentration SYBR Green Mix 2x 5.00 μl 21 105 μl 1x Primer mix (FWD/REV) 10μM 0.40 μl 21 8.4 μl 400nM (i.e. determined prior) H 2O (RNase/DNase free) 2.60 μl 21 54.6 μl N/A Total: 8.00 μl 168 μl For serial template (cDNA) dilutions: Commercial human cDNA (Clontech cat #636693) or experimental