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Interpretation of Nucleic Acid 260/280 Ratios

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nucleic acid absorbance at 260 nm is generally on a plateau, the absorbance curve at 280 nm is quite steeply sloped. A slight shift in wavelength accuracy will have a large effect on 260/280 ratios. It is possible to see as much as a 0.4 difference in the 260/280 ratio when measuring the same nucleic acid sample on two

  Interpretation, Acid, Nucleic acid, Nucleic, Interpretation of nucleic acid 260

Interpretation of Nucleic Acid 260/280 Ratios - always active

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T123 – TECHNICAL BULLETIN NanoDrop Lite Interpretation of Nucleic Acid 260/280 Ratios T123– Rev 1/2012 Thermo Scientific NanoDrop Products Wilmington, Delaware USA Technical support:nanodrop.techsupport@thermofisher.com

  Interpretation, Acid, Nucleic, Interpretation of nucleic acid 260

Interpretation of Nucleic Acid 260/280 Ratios

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sloped. A slight shift in wavelength accuracy will have a large effect on 260/280 ratios. It is possible to see as much as a 0.4 difference in the 260/280 ratio when measuring the same nucleic acid sample on two spectrophotometers that are both within a 1 nm wavelength accuracy specification. Reference . 1) William W. Wilfinger, Karol Mackey ...

  Interpretation, Acid, Nucleic, Sloped, Interpretation of nucleic acid 260

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