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1 IntroductiontoMassSpectrometry,aTutorial

11 Introduction to mass Spectrometry, a TutorialWilfried Niessen and David the past 30 years, mass spectrometry (MS) has undergone a spectacular devel-opment, in terms of both its technological innovation and its extent of applica-tion. On-line liquid chromatography mass spectrometry (LC MS) has becomea routine analytical tool, important in many application areas. The introductionof electrospray ionization (ESI) and matrix-assisted laser desorption/ionization(MALDI) has enabled the MS analysis of highly polar and large molecules, includ-ing biomacromolecules. MS is based on the generation of gas-phase analyte ions,the separation of these ions according to their mass -to-charge ratio (m/z), and thedetection of these ions.

1.2 Figures of Merit 5 obviouslydependsonthem/z value,thenumberofelementsconsidered,and the mass accuracy achieved [6]. The number of hits may also be reduced by taking an accurately measured isotope pattern of the ion into consideration

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