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10X Dilutions - Micrology Labs

10X DilutionsGenerally, an initial 10X dilution is prepared from the original sample. Further Dilutions arethen made from this initial dilution . In order to make this 10X dilution , follow the guidelinesbelow for sample size and amount of diluent to ProductsDairy ProductsAmt of sampleAmt of diluentAmt of sampleAmt of diluent10 mL or grams + 90 mL diluent11 mL or grams + 99 mL diluent50 mL or grams + 450 mL diluent55 mL or grams + 495 mL diluentSolid samples: Blend the sample with the diluent in a sterile blender for 2minutes, or massage the sample with the diluent in a stomacher bag for samples: Add the sample to the diluent in a sterile container (such as adiluent bottle or stomacher bag) and shake approximately 25 1 mL from this 10X dilution and place in the Easygel bottle.

10X Dilutions Generally, an initial 10X dilution is prepared from the original sample. Further dilutions are then made from this initial dilution.

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