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2.2.46. CHROMATOGRAPHIC SEPARATION TECHNIQUES …

Supercritical fluid chromatographyEUROPEAN PHARMACOPOEIA solutions. In addition to theTOC water control,prepare suitable blank solutions or other solutions neededfor establishing the baseline or for calibration adjustmentsfollowing the manufacturer s instructions; run the suitability. Run the following solutions and recordthe responses:TOC water(rw);standard solution(rs);systemsuitability solution(rss). Calculate the percentage responseefficiency using the expression:Thesystemissuitableiftherespo nseefficiencyisnotlessthan85 per cent and not more than 115 per cent of the (ru).The test solution complies with the test ifruis not greater thanrs method can also be applied using on-line instrumentationthat has been adequately calibrated and shown to haveacceptable system suitability.

separation methods in which the components of a sample are distributed between 2 phases, one of which is stationary, while the other is mobile. The stationary phase may be a solid or a liquid supported on a solid or a gel. The stationary phase may be packed in a column, spread as a layer, or distributed as a film, etc. The mobile phase may be ...

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