Transcription of 病原体検出マニュアル 2019-nCoV Ver.2
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病原体検出マニュアル 2019-nCoV Ver.2
1 2019-nCoV 2 2 17 HP 2019-nCoV 2 2 5 2 2 13 2 2 15 2019-nCoV open reading flame 1a (ORF1a) spike (S) 2-step RT-PCR TaqMan one-step RT-PCR 2019-nCoV 1 2019-nCoV ( ) HP 2 RNA QIAamp Viral RNA Mini Kit RNA 1) 2) QIAamp Viral RNA Mini Kit (QIAGEN ) Distilled water (Deionized, Sterile, autoclaved, DNase free, RNase free Cat No.
類似品(PrimeScript RT reagent Kit, Takara RR037A等)]、[Quick Taq HS Dymix Toyobo DTM-101または類似品(PerfectShot Ex Taq, Takara RR005A等)]、2019-nCoV特異的プ ライマー、Oligo(dT)12–18 Primer (Thermo, 18418012または類似品)、Random Hexamers (Thermo, N8080127、または類似品。
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