Transcription of 3 UV-VISIBLE SPECTROSCOPY - cffet.net
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3 UV-VISIBLE SPECTROSCOPY - cffet.net
3 UV-VISIBLE SPECTROSCOPY . Introduction Both molecular and atomic species can absorb radiation in the ultraviolet- visible (UV/VIS) regions of the electromagnetic spectrum by the absorption of energy by certain electrons in a substance. In this case, the ultraviolet region is considered to be above 200 nm. Different types of electrons, but not all types, can absorb UV/VIS radiation, depending on the type of substance, as shown in Table TABLE Types of electrons and their absorption behaviour Type of substance Type of electron Absorbs? Ion Inner shell No Outer shell (valence) Yes Covalently-bonded species Single bond No Double/triple bond Yes Unbonded Yes EXAMPLE Which of the following substances absorb UV/VIS radiation? hexane, hexene, trichloromethane, potassium chloride, iron (III) chloride, potassium nitrate Substance Absorb?
3. UV-Visible Spectroscopy Sci Inst Analysis (Spectro/Chrom) 3.3 These filter photometer are almost always single-beam, where the instrument is zeroed with a blank solution, and calibrated to 100% absorbance by shutting off the light path.
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