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4 Lentivirus Concentrator Solution

4 Lentivirus Concentrator Solution Dissolve 80g PEG-8000, NaCl in 80ml MillQ water and 20ml of 10 PBS ( ), Mix with gentle stirring, heating gently if necessary, until the solids are dissolved then adjust pH to ~ and the final volume to 200ml. Sterilize by filtering through M. The concentrations of PEG-8000 and NaCl in the stock Solution are 40% (W/V) and , respectively. Store the Solution at 4 oC. Virus concentration protocol [See Note 1]: 1. Collect supernatant from 10-cm culture dish into a 15 ml tube; 2. Spin down at 800 g for 10 min at room temperature or through a sterile m syringe filter ( Millipore # SLHV033RS); 3.

Lo HL, Yee JK. Production of Vesicular Stomatitis Virus G Glycoprotein (VSV-G) Pseudotyped Retroviral Vectors. In Current Protocols in Human Genetics, 2007 John Wiley & Sons, Inc. pp12.7.1-12.7.11;

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