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4 STERILISATION AND DISINFECTION

MICROBIOLOGYMODULES terilisation and DisinfectionMicrobiology 40 Notes4 STERILISATION INTRODUCTIOND isinfection and sterilization are essential for ensuring that medical and surgicalinstruments do not transmit infectious pathogens to patients. Because sterilizationof all patient-care items is not necessary, health-care policies must identify,primarily on the basis of the items intended use, whether cleaning, DISINFECTION ,or sterilization is reading this lesson, you will be able to:zdefine terms related to Sterilization and Disinfectionzclassify items to be sterilised or disinfectedzdiscuss different Methods of sterilisationzdescribe Evaluation and in Process Monitoring of Sterilization Procedureszdiscuss Methods of disinfectionzdescribe the Testing of DEFINITIONS OF TERMSS terilization: Sterilization describes a process that destroys or eliminates allforms of microbial life and is carried out in health-care facilities by physical orchemical methods.

the sterilization of laboratory glassware, media, and reagents. For efficient heat transfer, steam must flush the air out of the autoclave chamber. Before using the autoclave, check the drain screen at the bottom of the chamber and clean if blocked. If the sieve is blocked with debris, a layer of air may form at the bottom

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