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61 MICROBIOLOGICAL EXAMINATION OF NONSTERILE

56 55 Biological Indicators Resistance Performance Tests / MICROBIOLOGICAL TestsUSP 35 Viable Spore Count. D-value determination methods for pa-per carrier biological indicators may be used to calculate the 61 MICROBIOLOGICALD value for nonpaper carriers. Incubation conditions for themicroorganisms that may be used for nonpaper biologicalEXAMINATION OF NONSTERILE indicators are described in the Total Viable Spore :MICROBIALFor Biological Indicators for Moist Heat, Dry Heat, and Gase-ous Modes of Sterilization, Liquid Spore Suspensions, theENUMERATION TESTS method of recovery following sterilization exposure condi-tions are those methods described in the Total Viable SporeCount section for liquid suspensions, and when a dry heatD-value determination is made from B. atrophaeus suspen-sions, the same recovery procedures as described under Bio-INTRODUCTION logical Indicator for Steam Sterilization, Paper Carrier tests described hereafter will allow quantitative enu-Where C.

Aspergillus brasiliensis such Sabouraud Dextrose Agar Soybean–Casein Sabouraud Soybean–Casein Sabouraud as ATCC 16404, IMI or Potato–Dextrose Agar Digest Agar Dextrose Agar Digest Agar Dextrose Agar 149007, IP 1431.83, or 20°–25° 5–7 days, or ≤100 cfu ≤100 cfu ≤100 cfu ≤100 cfu

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  Agar, Potato, Potato dextrose agar, Dextrose, Dextrose agar, Agar dextrose agar

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