Transcription of 9221 MULTIPLE-TUBE FERMENTATION ... - Edge Analytical
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9221 MULTIPLE-TUBE FERMENTATION ... - Edge Analytical
9221 MULTIPLE-TUBE FERMENTATION TECHNIQUE FOR MEMBERS OF THECOLIFORM GROUP*9221 A. IntroductionThe coliform group consists of several genera of bacteriabelonging to the family Enterobacteriaceae. The historical defi-nition of this group has been based on the method used fordetection, lactose FERMENTATION , rather than on the tenets ofsystematic bacteriology. Accordingly, when the fermentationtechnique is used, this group is defined as all facultative anaer-obic, gram-negative, non-spore-forming, rod-shaped bacteriathat ferment lactose with gas and acid formation within 48 h at35 standard test for the coliform group may be carried out bythe MULTIPLE-TUBE FERMENTATION technique or presence absenceprocedure (through the presumptive-confirmed phases or com-pl)
ining drinking water via the fermentation technique, process all tubes or bottles demonstrating growth, with or without a positive acid or gas reaction, to the confirmed phase (9221B.3). Drinking water samples that are positive for total coliforms also must be tested for thermotolerant (fecal) coliforms (9221E) or Esche-richia coli (9221F).
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