9221 MULTIPLE-TUBE FERMENTATION TECHNIQUE FOR …

9221 MULTIPLE-TUBE FERMENTATION TECHNIQUE FOR MEMBERS OF THECOLIFORM GROUP*9221 A. IntroductionThe coliform group consists of several genera of bacteriabelonging to the family Enterobacteriaceae. The historical defi-nition of this group has been based on the method used fordetection, lactose FERMENTATION , rather than on the tenets ofsystematic bacteriology. Accordingly, when the fermentationtechnique is used, this group is defined as all facultative anaer-obic, gram-negative, non-spore-forming, rod-shaped bacteriathat ferment lactose with gas and acid formation within 48 h at35 standard test for the coliform group may be carried out bythe MULTIPLE-TUBE FERMENTATION TECHNIQUE or presence absenceprocedure (through the presumptive-confirmed phases or com-pleted test) described herein, the membrane filter (MF) TECHNIQUE ( section 9222) or the enzymatic substrate coliform test (Section9223).

(Section 9222) or the enzymatic substrate coliform test (Section 9223). Each technique is applicable within the limitations spec-ified and with due consideration of the purpose of the examina-tion. Production of valid results requires strict adherence to quality control procedures. Quality control guidelines are out-lined in Section 9020.

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