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A method for preparing 2- to 50-µm-thick ... - …
AbstractThis article describes a method for preparing2- to 50- m- thick fresh - frozen sections from large sam-ples and completely calcified tissue samples. In order toperform the more routine work involved, a tungsten car-bide disposable blade was installed to a heavy-dutysledge cryomicrotome. An entire 10-day-old rat andbone and tooth samples from a 7-month-old rat were rap-idly frozen . The frozen samples were attached to thecryomicrotome stage. The cutting surface of the sampleswas covered with a polyvinylidene chloride film coatedwith synthetic rubber cement and cut at 25 C. The softtissues and the hard tissues were satisfactorily preservedand all tissue cells were easily identifiable. Enzymaticactivity in the fresh sections was much stronger than thatin chemically fixed and/or decalcified sections.
Abstract This article describes a method for preparing 2- to 50-µm-thick fresh-frozen sections from large sam-ples and completely calcified tissue samples.
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