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RJCES Volume 1 [1] April 2013 2 | P a g e A Simple and Rapid HPLC Method for the Simultaneous Determination of Vitamin-A and Vitamin-E in Tablet Dosage Forms Kareti Srinivasa Rao*a, Nargesh K. Keshar a, Nikhil V Belorkar a , Bhanoji Raoa, K Srinivasab and Ajay Kumar Pattnaikc a Roland Institute of Pharmaceutical Sciences, Berhampur, Orissa-760 010 bSri Vasavi Institute of Pharmaceutical Siences, Tadepalligudem 534 101, cRavenshaw University, Cuttack, Odisha ABSTRACT A Simple and precise high performance liquid chromatographic Method has been developed and validated for the simultaneous determination of vitamin A ( ) and vitamin E ( ) in bulk and pharmaceutical dosage forms. Chromatography was carried out at ambient temperature on a 150mm, 5 m Altech prevail-C18 column with mobile phase acetonitrile and methanol (75:25) at a flow rate of ml/min. The UV detection was carried out at 220 nm.
RJCES Volume 1 [1] April 2013 www.aelsindia.com 3 | P a g e reported method describes simple and rapid LC method for simultaneous determination of vit.A …
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