A simple electroporation method of green fluorescent ...

A simple electroporation method of green fluorescent protein-transfection and in vitro imaging of organ-cultured embryonic lingual tissue K. Yoshimura*1,T. Nashida2, M. Mikami3 and I. Kageyama1 1 Department of Anatomy, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Niigata 951-8580, Japan 2 Department of Biochemistry, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Niigata 951-8580, Japan 3 Department of Microbiology, The Nippon Dental University School of Life Dentistry at Niigata, 1-8 Hamaura-cho, Niigata 951-8580, Japan We aimed to establish a technique for efficiently transfecting embryonic organ-cultured lingual tissue using electroporation . To visually evaluate the transfection in the organ-cultured tissue, we use the constructed pCAGGS-eGFP vector, which expresses green fluorescent protein (GFP).

Fig. 5 A set of macroscopic views of intact lingual tissue and in vitro imaging of the eGFP fluorescence at 24, 48, and 72 h post- transfection in experiment 2. Arrowheads: eGFP-transfected area. Scale bars = 2.0 mm In experiment 3, we configured the number of transfer pulses to be fixed at 30 and adjusted the pulse voltages to be

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  Imaging, Fluorescence

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