Transcription of Affinity Chromatography Principles and Methods Handbook
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Affinity Chromatography Principles and Methods Handbook
GE Healthcare Affinity Chromatograpy Handbook Principles and Methods GE, imagination at work and GE Monogram are trademarks of General Electric Company. KTA, KTAexplorer, KTAFLPC, KTAprime, KTApurifier, Biacore, BioDirectory, BioProcess, ECL, ECL Plus, ExcelGel, FPLC, GSTPrep, GSTrap, HisTrap, HiPrep, HiTrap, Hybond, MAbTrap, MabSelect, MicroSpin, Microplex, Multiphor, STREAMLINE, Sepharose, Percoll, PhastSystem, PhastGel, Sephadex, Superdex, and Tricorn are trademarks of GE Healthcare companies. Purification and preparation of fusion proteins and Affinity peptides comprising at least two adjacent histidine residues may require a license under US pat 5,284,933. and US pat 5,310,663, including corresponding foreign patents (assigne: Hoffman La Roche, Inc). A license for commercial use of GST gene fusion vectors must be obtained from Chemicon International, Incorprated, 28820 Singel Oak Drive, Temecula, California 92590 USA.
an efficient multi-step process must be developed using the purification strategy of Capture, Intermediate Purification and Polishing (Cipp). When applying this strategy affinity chromatography offers an ideal capture or intermediate step in any purification protocol and can be used whenever a suitable ligand is available for the protein of ...
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