Transcription of Affinity Chromatography Principles and Methods Handbook
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Affinity Chromatography Principles and Methods Handbook
GE Healthcare Affinity Chromatograpy Handbook Principles and Methods GE, imagination at work and GE Monogram are trademarks of General Electric Company. KTA, KTAexplorer, KTAFLPC, KTAprime, KTApurifier, Biacore, BioDirectory, BioProcess, ECL, ECL Plus, ExcelGel, FPLC, GSTPrep, GSTrap, HisTrap, HiPrep, HiTrap, Hybond, MAbTrap, MabSelect, MicroSpin, Microplex, Multiphor, STREAMLINE, Sepharose, Percoll, PhastSystem, PhastGel, Sephadex, Superdex, and Tricorn are trademarks of GE Healthcare companies. Purification and preparation of fusion proteins and Affinity peptides comprising at least two adjacent histidine residues may require a license under US pat 5,284,933.
Charge Ion exchange chromatography Size Gel filtration (sometimes called size exclusion) Hydrophobicity Hydrophobic interaction chromatography Reversed phase chromatography Fig. 1. Separation principles in chromatographic purification. Affinity chromatography separates proteins on the basis of a reversible interaction between
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