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An evaluation of the Ceriodaphnia dubiachronic toxicity ...

Margaret Passmore, Amy Bergdale, Greg Pond, Louis Reynolds, Kelly Krock and Frank Borsuk US EPA R3, Wheeling, WVAn evaluation of the Ceriodaphnia dubiachronic toxicity test as an indicator of instream effects from mountaintop coal miningJones BranchMiddle Fork WatershedMartin Co., KYBackground Alkaline coal mining effluent Elevated Total Dissolved Solids (dominated by Ca2+, Mg2+, HCO3- and SO42-), Fe, Mn, Ni, Zn, Se and NO3- Mined streams have increased base flow Adverse effects to water quality and benthic macroinvertebratesBackground NPDES permit limits require pH, Fe and TSS Some WQBELs for Mn, Al, Se No limits for TDS/conductivity/ions Whole Effluent toxicity (WET) testing has been proposed for NPDES permits Would WET protect native biota?Objectives What level of water quality degradation is associated with lethal or sublethal effects to C. dubia? What s causing the observed toxicity ?

Background • NPDES permit limits require pH, Fe and TSS • Some WQBELs for Mn, Al, Se • No limits for TDS/conductivity/ions • Whole Effluent Toxicity (WET) testing

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  Evaluation, Testing, Toxicity, Ceriodaphnia, Dubiachronic, Evaluation of the ceriodaphnia dubiachronic toxicity

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