Transcription of An Introduction to Fluorescence Polarization - Polito
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The theory of Fluorescence Polarization , first described in 1926 by Perrin, is based on theobservation that fluorescent molecules in solution, excited with plane-polarized light, will emit lightback into a fixed plane ( the light remains polarized) if the molecules remain stationary during theexcitation of the fluorophore. Molecules, however, rotate and tumble, and the planes into which lightis emitted can be very different from the plane used for initial Polarization of a molecule is proportional to the molecule`s rotational relaxation time, or the timeit takes to rotate through an angle of . Rotational relaxation time is related to viscosity, absolutetemperature, molecular volume and the gas : When a fluorescent molecule is excited with plane polarized light, light is emitted in thesame polarized plane, provided that the molecule remains stationary throughout the excited state(which has a duration of 4 nanoseconds for fluorescein).
The theory of Fluorescence Polarization, first described in 1926 by Perrin, is based on the observation that fluorescent molecules in solution, excited with plane-polarized light, will emit light back into a fixed plane (i.e. the light remains polarized) if the molecules remain stationary during the
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Introduction to Fluorescence, INTRODUCTION, Fluorescence Microscopy, Introduction to Fluorescence Microscopy, Fluorescence Imaging, FLUORESCENCE, Introduction Fluorescence, Lecture Notes: Introduction to Spectroscopy, Introduction to Fluorescence and Phosphorescence, INTRODUCTION TO ENERGY-DISPERSIVE X, Introduction Fluorescence Microscopy, X-Ray Fluorescence