Transcription of Analysis of -Carotene in Carrot by Spectrophotometry
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Analysis of -Carotene in Carrot by Spectrophotometry
Analysis of -Carotene in Carrot by Spectrophotometry P. Karnjanawipagul, W. Nittayanuntawech, P. Rojsanga and L. Suntornsuk* Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University Abstract This project aimed to develop a simple UV spectrophotometric method for the Analysis of -carotene in Carrot . Extraction of -carotene from Carrot was simply by liquid-liquid extraction and UV absorbance was measured at 461 nm. The developed method was valid for its linearity, accuracy, precision, limit of detection (LOD) and limit of quantitative (LOQ). The UV spectrophotometric method illustrated excellent linearity (r2 = ) in a range of 1-8 g/mL. Precision was good with relative standard deviation of less than and average recovery was The LOD of UV spectrophotometric measurement was g/mL and the LOQ was g/mL.
were β-carotene concentration and UV absorption at 461 nm, respectively. Later the linearity range was increased from 1-8 µg/mL since carrot might contain β-carotene
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