Transcription of Analysis of Relative Gene Expression Data Using …
{{id}} {{{paragraph}}}
Example: confidence
Analysis of Relative Gene Expression Data Using …
METHODS 25, 402 408 (2001). , available online at on Analysis of Relative gene Expression data Using real - Time Quantitative PCR and the 2 CT Method Kenneth J. Livak* and Thomas D. Schmittgen ,1. *Applied Biosystems, Foster City, California 94404; and Department of Pharmaceutical Sciences, College of Pharmacy, Washington State University, Pullman, Washington 99164-6534. of the target gene Relative to some reference group The two most commonly used methods to analyze data from real -time, quantitative PCR experiments are absolute quantifica- such as an untreated control or a sample at time zero tion and Relative quantification. Absolute quantification deter- in a time-course study. mines the input copy number, usually by relating the PCR signal Absolute quantification should be performed in situ- to a standard curve. Relative quantification relates the PCR signal ations where it is necessary to determine the absolute of the target transcript in a treatment group to that of another transcript copy number.
METHODS 25, 402–408 (2001) doi:10.1006/meth.2001.1262, available online at http://www.idealibrary.com on Analysis of Relative Gene Expression Data Using Real-
Tags:
Information
Domain:
Source:
Link to this page:
Please notify us if you found a problem with this document: