Transcription of Appendix IV (B): Chromatography – High …
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Appendix IV (B): Chromatography – High …
Appendix IV(B) Chromatography high - performance liquid Chromatography (HPLC) Appendix IV (B): Chromatography high - performance liquid Chromatography (HPLC)HPLC is a separation technique consisting of a solid stationary phase and a liquid mobile phase. The sampleis injected through an injector and carried into the column by the mobile phase, the components are separatedon the stationary phase and pass through the detector in succession, a chromatogram is recorded.(1)Preparation of test sample Powder the CMM sample and pass through a sieve before quantity of the sample to be powdered should be of at least five times as much as those needed forthe analysis.(2)General requirements for the apparatus Set up the stationary and mobile phases of the HPLC asspecified in the individual monograph. One of the most commonly used packing material is ODSchemically bonded to silica. Ion exchange resins are used for ion exchange Chromatography and poroussilica or polymers are used for size exclusion Chromatography .
Appendix IV(B) Chromatography – High-Performance Liquid Chromatography (HPLC) Appendix IV (B): Chromatography – High-Performance Liquid Chromatography (HPLC)
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