Transcription of Aseptic Technique for Cell Culture UNIT 1 - CPP
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Aseptic Technique for Cell Culture UNIT 1 - CPP
UNIT Technique for Cell CultureThis unit describes some of the ways that a laboratory can deal with the constant threatof microbial contamination in cell cultures. Microorganisms are ubiquitous. Bacteria canbe isolated from nearly any surface including inanimate objects and human skin. Fungalspores and bits of vegetative hyphae drift into a laboratory from air conditioning ductsand open doors. Mycoplasma infections most frequently originate from improperlysterilized media or serum. At the risk of eliciting paranoia in the novice cell Culture userwho has no training in microbiological techniques, the possibility for microbial contami-nation exists everywhere.
In reality, aseptic technique cannot be presented in one easily outlined protocol, but rather encompasses all aspects of environ-mental control, personal hygiene, equipment and media sterilization, and associated quality control procedures needed to ensure that a procedure is, indeed, performed with aseptic, noncontaminating technique.
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