Transcription of Aseptic Technique for Cell Culture UNIT 1 - CPP
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Aseptic Technique for Cell Culture UNIT 1 - CPP
UNIT Technique for Cell CultureThis unit describes some of the ways that a laboratory can deal with the constant threatof microbial contamination in cell cultures. Microorganisms are ubiquitous. Bacteria canbe isolated from nearly any surface including inanimate objects and human skin. Fungalspores and bits of vegetative hyphae drift into a laboratory from air conditioning ductsand open doors. Mycoplasma infections most frequently originate from improperlysterilized media or serum. At the risk of eliciting paranoia in the novice cell Culture userwho has no training in microbiological techniques, the possibility for microbial contami-nation exists everywhere.
The horizontal laminar-flow clean bench is used to provide a near-sterile environment for the clean (i.e., noncontaminating) handling of nonhazardous material such as sterile media or equipment. Because the air stream pattern directs the flow of air within the hood directly back to the hood operator and the room (Fig. 1.3.1), horizontal flow ...
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