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ASSAY DEVELOPMENT IN DRUG DISCOVERY

ASSAY DEVELOPMENT IN DRUG DISCOVERYI ndira Padmalayam, DISCOVERY Division, Southern Research Institute, Birmingham, Alabama 35205 The Drug DISCOVERY Process:The ASSAY DEVELOPMENT StageAssay developmentPrimary assays Secondary assaysTarget IdentificationTarget ValidationAssayDevelopmentHTSLead IdentificationLead OptimizationDevelopment Hit validationChemistryStructure-Activity Relationship (SAR)bioavailability (PK, ADME), toxicityIn vivo efficacyPre-clinicalGLP-ToxClinicalTarge t IdentificationTarget ValidationAssayDevelopmentLead Optimization DevelopmentHTSNon-HTSA ssay DEVELOPMENT .

Assay development: A critical part of the “hit” discovery process ... MDA-MB-231 breast cancer cells were co-cultured with 3T3L1 fibroblasts and treated with Cytochalasin-D, an inhibitor of actin polymerization. Migration of cells through the matrigel layer was measured at …

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