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Automated DNA Sequencing - Applied Biosystems

Automated DNA Sequencing Chemistry Guide Copyright 2000, Applied Biosystems For Research Use Only. Not for use in diagnostic procedures. ABI PRISM and its design, Applied Biosystems , and MicroAmp are registered trademarks of Applera Corporation or its subsidiaries in the andcertain other , BigDye, CATALYST, POP, POP-4, POP-6, and Primer Express are trademarks of Applera Corporation or its subsidiaries in the and certainother , AmpliTaq Gold, and GeneAmp are registered trademarks of Roche Molecular Systems, is a registered trademark of W. R. Grace and is a trademark of Princeton Separations, Ranger is a trademark of The FMC and Power Macintosh are registered trademarks of Apple Computer, is a registered trademark of Promega Corporation. Contents i 1 Introduction.. 1-1 New DNA Sequencing Chemistry Guide .. 1-1 Introduction to Automated DNA Sequencing .

Introduction 1-3 Fluorescent Sequencing In the Applied Biosystems strategy for automated fluorescent sequencing, fluorescent dye labels are incorporated into DNA extension products using 5´-dye labeled primers

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