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Automated DNA Sequencing - Applied Biosystems

Automated DNA Sequencing Chemistry Guide Copyright 2000, Applied Biosystems For Research Use Only. Not for use in diagnostic procedures. ABI PRISM and its design, Applied Biosystems , and MicroAmp are registered trademarks of Applera Corporation or its subsidiaries in the andcertain other , BigDye, CATALYST, POP, POP-4, POP-6, and Primer Express are trademarks of Applera Corporation or its subsidiaries in the and certainother , AmpliTaq Gold, and GeneAmp are registered trademarks of Roche Molecular Systems, is a registered trademark of W. R. Grace and is a trademark of Princeton Separations, Ranger is a trademark of The FMC and Power Macintosh are registered trademarks of Apple Computer, is a registered trademark of Promega Corporation. Contents i 1 Introduction.. 1-1 New DNA Sequencing Chemistry Guide .. 1-1 Introduction to Automated DNA Sequencing .. 1-2 ABI P RISM Sequencing Chemistries .. 1-5 Applied Biosystems DNA Sequencing Instruments.

1-2 Introduction Introduction to Automated DNA Sequencing Sanger Dideoxy Sequencing DNA polymerases copy single-stranded DNA

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